Two-Dimensional NMR of Macromolecules

Two-dimensional (2D) NMR is a versatile technique which exists in many versions. Two broad classes of 2D techniques are (1) correlated spectroscopy and (2) J-resolved spectroscopy. The first of these may be divided into two further subdivisions: COSY, which permits correlations of resonances via J coupling, and NOESY, which allows direct measurement of internuclear (usually interproton) distances by the nuclear Overhauser effect. COSY greatly facilitates the interpretation of complex spectra and spectral interpretation in terms of stereochemical sequences is placed on a firmer foundation.