The Two Dimensional NMR Spectroscopy of Macromolecules.

Two-dimensional (2D) NMR is a versatile technique which exists in many versions. Two broad classes of 2D techniques are (1) correlated spectroscopy and (2) J-resolved spectroscopy. The first of these may be divided into two further subdivisions: COSY, which permits correlations of resonances via J coupling, and NOESY, which allows direct measurement of internuclear (usually interproton) distances by the nuclear Overhauser effect. COSY greatly facilities the interpretation of complex spectra and spectral interpretation in terms of stereochemical sequences is placed on a firmer foundation. NOESY provides direct information concerning the local conformations of polymers in solution.